- Title
- Docosahexaenoic acid inhibits the invasion of MDA-MB-231 breast cancer cells through upregulation of cytokeratin-1
- Creator
- Blanckaert, Vincent; Kerviel, Vincent; Lépinay, Alexandra; Joubert-Durigneux, Vanessa; Hondermarck, Hubert; Chénais, Benoît
- Relation
- International Journal of Oncology Vol. 46, Issue 6, p. 2649-2655
- Publisher Link
- http://dx.doi.org/10.3892/ijo.2015.2936
- Publisher
- Spandidos Publications
- Resource Type
- journal article
- Date
- 2015
- Description
- Docosahexaenoic acid (DHA), the main member of the omega-3 essential fatty acid family, has been shown to reduce the invasion of the triple-negative breast cancer cell line MDA-MB-231, but the mechanism involved remains unclear. In the present study, a proteomic approach was used to define changes in protein expression induced by DHA. Proteins from crude membrane preparations of MDA-MB-231 cells treated with 100 µM DHA were separated by two-dimensional electrophoresis (2-DE) and differentially expressed proteins were identified using MALDI-TOF mass spectrometry. The main changes observed were the upregulation of Keratin, type II cytoskeletal 1 (KRT1), catalase and lamin-A/C. Immunocytochemistry analyses confirmed the increase in KRT1 induced by DHA. Furthermore, in vitro invasion assays showed that siRNA against KRT1 was able to reverse the DHA-induced inhibition of breast cancer cell invasion. In conclusion, KRT1 is involved in the anti-invasive activity of DHA in breast cancer cells.
- Subject
- breast cancer; cytokeratin-1; docosahexaenoic acid; omega-3; tumor cell invasion
- Identifier
- http://hdl.handle.net/1959.13/1338067
- Identifier
- uon:27950
- Identifier
- ISSN:1791-2423
- Language
- eng
- Full Text
- Reviewed
- Hits: 4908
- Visitors: 5439
- Downloads: 379
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 533 KB | Adobe Acrobat PDF | View Details Download |